23b000a1 P52L:T57S:T60S:H146R:N157D: log10 fluorescence 3.136 (robust z -0.01) — no change
walkthrough · branch plate-1-c5 · 2026-10-29 18:40 UTC
Observation failure
| instrument | BMG CLARIOstar |
|---|---|
| capture source |
pasted_export
pasted from the instrument's own export
|
Raw artifact, stored verbatim:
{"is_hit": false, "label": "P52L:T57S:T60S:H146R:N157D", "measurement": "log10 fluorescence", "parent": "15efe7753aa99b60a8d8afaabbbfce7a42d8d54d", "robust_z": -0.005807551091855929, "screen": "plate-1", "value": 3.13625898055, "well": "C5"}
Strain fields below are inherited from the parent record — an observation reports on a strain, it does not change it.
Record
| genotype | |
|---|---|
| marker | |
| phenotype | |
| protocol | |
| strain | |
| full sha | 23b000a1eb1630640b82e9ba4a65b8363e971730 |
Barcode (format v0)
GGTCTCAAGATGTAAAAAAGGACGTTGAGACC32 nt · structure: 7 nt anchor + 16 nt payload (first 8 hex chars of the commit sha at 2 bits/base) + 2 nt checksum + 7 nt anchor. Integrate at a neutral genomic site; any single-base read error in the core is detected by the checksum.
GC 44% max homopolymer 6 no restriction sites in core
Constraint warnings are informational in v0 — the commit id is fixed by content, so a failing barcode flags a candidate for re-encoding strategies (a v1 concern).