lacz-knockout-k12
Commit history (all branches, newest first)
Observations recorded: 1 success — failures are kept permanently, not discarded.
| Commit | Branch | Message | Genotype / observation | Author | Time |
|---|---|---|---|---|---|
583864a1
|
main | success qPCR: 3 of 8 clones positive | QuantStudio 5 · pasted_export | t.meany | 2026-10-29 11:08 UTC |
c003c49e
|
main | P_tet-sfGFP integrated at attB neutral site | dlacZ::kanR, attB::P_tet-sfGFP | l.hobbs | 2026-10-29 09:40 UTC |
18fa6469
|
marker-cured | FLP excision of kanR cassette | dlacZ (scar) | t.meany | 2026-10-29 09:40 UTC |
7c7082e1
|
main | lacZ knockout via lambda-Red recombineering | dlacZ::kanR | t.meany | 2026-10-29 09:40 UTC |
2e125e25
|
main | Initial record: wild-type base strain | F- lambda- ilvG- rfb-50 rph-1 | l.hobbs | 2026-10-29 09:40 UTC |
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